Deoxynivalenol-induced cytokines and related genes in concanavalin A-stimulated primary chicken splenic lymphocytes

Zhihua Ren; Yachao Wang; Huidan Deng; Youtian Deng; Junliang Deng; Zhicai Zuo; Ya Wang; Xi Peng; Hengmin Cui; Liuhong Shen; Xiaoping Ma; Jing Fang

doi:10.1016/j.tiv.2014.12.006

Deoxynivalenol-induced cytokines and related genes in concanavalin A-stimulated primary chicken splenic lymphocytes

Toxicol In Vitro. 2015 Apr;29(3):558-63. doi: 10.1016/j.tiv.2014.12.006. Epub 2014 Dec 24.

Authors

Zhihua Ren¹, Yachao Wang², Huidan Deng³, Youtian Deng⁴, Junliang Deng⁵, Zhicai Zuo⁶, Ya Wang⁷, Xi Peng⁸, Hengmin Cui⁹, Liuhong Shen¹⁰, Xiaoping Ma¹¹, Jing Fang¹²

Affiliations

¹ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: zhihua_ren@126.com.
² College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China. Electronic address: wangyachao1688@126.com.
³ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: 847767028@qq.com.
⁴ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: dengyt1012@hotmail.com.
⁵ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: dengjl213@126.com.
⁶ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: zzcjl@126.com.
⁷ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: wangyadudu@126.com.
⁸ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: pengxi197313@163.com.
⁹ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: cui580420@sicau.edu.cn.
¹⁰ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: shenliuhong@sohu.com.
¹¹ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: mxp886@sina.com.
¹² College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: fangjing4109@163.com.

PMID: 25542652
DOI: 10.1016/j.tiv.2014.12.006

Abstract

Deoxynivalenol (DON) immunotoxicity and its induction of cytokines and related genes in the splenic lymphocytes of chickens have not been completely elucidated. In the present study, we aimed to evaluate the effects of 48 h of different DON treatments (0 μg/mL, 0.2 μg/mL, 0.8 μg/mL, 3.2 μg/mL, 12.5 μg/mL, and 50 μg/mL) on the secretion and the mRNA expressions of some cytokine genes, such as interleukin-1beta (IL-1β), IL-1RI, IL-2, IL-4, IL-6, IL-10, IL-12β, and IFN-γ in chicken splenic lymphocytes. The concentrations of IL-1RI, IL-6, IL-10, and IFN-γ were increased with the DON concentrations increasing (P<0.05 or P<0.01). However, the concentrations of IL-1β, IL-2, IL-4, and IL-12β were decreased with the DON concentrations increasing (P<0.05 or P<0.01). Except IL-1β, the mRNA expressions of the other cytokines were up-regulated by DON. The highest mRNA expressions values of IL-1RI, IL-4, IL-10, IL-12β, and IFN-γ were at 50 μg/mL DON treatment groups (P<0.05 or P<0.01), while the highest mRNA expressions values of IL-2 and IL-6 were at 12.5 μg/mL DON treatment groups (P<0.05 or P<0.01). Our data revealed that the potent effects of DON in affecting the secretion and the mRNA expression of the related cytokines in chicken splenic lymphocytes in vitro.

Keywords: Chicken; Cytokines; Deoxynivalenol; Immunity; Mycotoxin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens / physiology*
Concanavalin A / pharmacology*
Cytokines / biosynthesis*
Lymphocytes / drug effects*
Male
Primary Cell Culture
Spleen / cytology
Spleen / drug effects
Trichothecenes / toxicity*

Substances

Cytokines
Trichothecenes
Concanavalin A
deoxynivalenol