Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells

FEBS Lett. 2015 Jan 30;589(3):310-8. doi: 10.1016/j.febslet.2014.12.013. Epub 2014 Dec 24.

Abstract

Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.

Keywords: Adenosine triphosphate; Low-intensity pulsed ultrasound; Osteogenesis; P2X7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / genetics
  • Adenosine Triphosphate / metabolism
  • Alkaline Phosphatase / metabolism
  • Animals
  • Cell Differentiation / genetics*
  • Cell Differentiation / radiation effects
  • Cell Proliferation / radiation effects
  • Humans
  • Mice
  • Osteoblasts / metabolism
  • Osteoblasts / radiation effects
  • Osteogenesis / genetics*
  • Osteogenesis / radiation effects
  • Receptors, Purinergic P2X7 / genetics
  • Receptors, Purinergic P2X7 / metabolism*
  • Sound*

Substances

  • Receptors, Purinergic P2X7
  • Adenosine Triphosphate
  • Alkaline Phosphatase