Complete mitochondrial genome of Sebastes owstoni (Scorpaenidae, Scorpaeniformes) from the East Sea, Korea

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):3952-3954. doi: 10.3109/19401736.2014.989501. Epub 2014 Dec 26.

Abstract

Sebastes owstoni is a popular fishery species in the north-western Pacific Ocean. To obtain basic information on its mitochondrial DNA (mtDNA), the complete mitogenome of S. owstoni was subjected to the next-generation sequencing. The assembled mtDNA was 16,465 bp in length, comprising 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs and a control region. The complete mitogenome comprised of 27.7% A, 26.6% T, 17.3% G and 28.4% C, indicating a slight AT bias (54.3%). The start codon of all PCGs was ATG, with the exception of COX1 (GTG). The stop codons in the PCGs were mainly TAA, with the exception of ND1 and ND3 (TAG), ND4 (AGA) and Cytb (incomplete termination codon, T). All tRNAs had a typical cloverleaf shape, with the exception of tRNASer (AGY), which lacked the DHU arm. The sequenced mitogenome of S. owstoni can be used in genetics-based management initiatives or for constructing the mitochondrial lineages within Scorpaenidae.

Keywords: Mitochondrial genome; Sebastes owstoni; Sebastinae; rockfish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Codon, Initiator
  • Codon, Terminator
  • DNA, Mitochondrial / chemistry
  • DNA, Mitochondrial / isolation & purification
  • DNA, Mitochondrial / metabolism
  • Electron Transport Complex IV / chemistry
  • Electron Transport Complex IV / genetics
  • Fish Proteins / chemistry
  • Fish Proteins / genetics
  • Genome, Mitochondrial*
  • High-Throughput Nucleotide Sequencing
  • Open Reading Frames / genetics
  • Perciformes / classification
  • Perciformes / genetics*
  • Phylogeny
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics
  • RNA, Transfer / chemistry
  • RNA, Transfer / genetics
  • Republic of Korea
  • Sequence Analysis, DNA

Substances

  • Codon, Initiator
  • Codon, Terminator
  • DNA, Mitochondrial
  • Fish Proteins
  • RNA, Ribosomal
  • RNA, Transfer
  • Electron Transport Complex IV