The objective of the work was to research the features of monoand combined impact of ionizing radiation and copper salts on cell viability in vitro. Materials and methods. The studies were performed on a passaged line L929 cell culture. Copper ions of different concentrations were added to the culture medium 1 hour after exposure to irradiation at doses of 0.5; 5.0 and 10.0 Gy. There were evaluated the structure of the cell populations, the proliferative and mitotic activity and apoptosis. Results and conclusions. After incubation of the cells with copper ions of various concentrations there were seen both the activation and inhibition of proliferative and mitotic activity. Cell survival and mitotic activity were decreased in exposure to increasing doses of radiation but the polykaryocyte index was increased. After the combined in vitro exposure to ionizing radiation and copper ions there were seen morphofunctional changes of cell properties, which were not in direct proportional dependence on the dose or the copper ions concentration.
Cel'yu raboty stalo issledovanie osobennostej otdel'nogo i kombinirovannogo dejstviya ioniziruyushhego izlucheniya i solej medi na zhiznesposobnost' kletok in vitro. Materialy i metody. Issledovaniya vypolneny na perevivaemoj kul'ture kletok linii L929. Iony medi v raznyx koncentraciyax dobavlyali v kul'tural'nuyu sredu cherez 1 chas posle ix oblucheniya v dozax 0,5; 5,0 i 10,0 Gr. Ocenivali strukturu populyacii kletok, ix proliferativnuyu i mitoticheskuyu aktivnost', apoptoz. Rezul'taty issledovanij i vyvody. Pri inkubacii kletok s ionami medi v razlichnyx koncentraciyax nablyudali kak aktivaciyu, tak i ingibirovanie proliferativnoj i mitoticheskoj aktivnosti kletok. Vyzhivanie kletok i mitoticheskaya aktivnost' pri obluchenii umen'shayutsya s uvelicheniem dozy, a indeks polikariocitov rastet. Pri kombinirovannom dejstvii ioniziruyushhego izlucheniya i ionov medi na kletki in vitro nablyudali izmeneniya morfofunkcional'nyx svojstv kletok, kotorye pryamo proporcional'no ne zaviseli ni ot dozy, ni ot koncentracii ionov medi.
Keywords: apoptosis; cell culture; heavy metals; ionizing radiation; proliferation; survival.
D. D. Hapeienko, H. I. Lavrenchuk, V. S. Asmolkova, V. N. Oksamytnyi.