Abstract
The radiolabelled platelet activating factor (PAF) receptor antagonist, [3H]WEB 2086, bound to specific sites on membrane fractions from homogenised guinea pig and human lungs. The sites on guinea pig and human membranes bound [3H]WEB 2086 with dissociation constants (KD) of 16.8 and 22.6 nM and binding capacities (Bmax) of 203 and 157 fmol/mg protein, respectively. In both species, binding was displaced competitively by PAF, suggesting that the sites labelled by [3H]WEB 2086 are PAF receptors.
MeSH terms
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Animals
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Azepines / pharmacology*
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Guinea Pigs
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In Vitro Techniques
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Lung / drug effects
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Lung / metabolism*
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Male
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Membranes / drug effects
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Membranes / metabolism
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Platelet Activating Factor / antagonists & inhibitors*
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Platelet Membrane Glycoproteins*
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Receptors, Cell Surface / metabolism*
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Receptors, G-Protein-Coupled*
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Triazines / pharmacology*
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Triazoles*
Substances
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Azepines
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Platelet Activating Factor
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Platelet Membrane Glycoproteins
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Receptors, Cell Surface
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Receptors, G-Protein-Coupled
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Triazines
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Triazoles
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platelet activating factor receptor
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WEB 2086