[3H]WEB 2086 labels platelet activating factor receptors in guinea pig and human lung

Eur J Pharmacol. 1989 Oct 10;169(2-3):313-6. doi: 10.1016/0014-2999(89)90029-0.

Abstract

The radiolabelled platelet activating factor (PAF) receptor antagonist, [3H]WEB 2086, bound to specific sites on membrane fractions from homogenised guinea pig and human lungs. The sites on guinea pig and human membranes bound [3H]WEB 2086 with dissociation constants (KD) of 16.8 and 22.6 nM and binding capacities (Bmax) of 203 and 157 fmol/mg protein, respectively. In both species, binding was displaced competitively by PAF, suggesting that the sites labelled by [3H]WEB 2086 are PAF receptors.

MeSH terms

  • Animals
  • Azepines / pharmacology*
  • Guinea Pigs
  • In Vitro Techniques
  • Lung / drug effects
  • Lung / metabolism*
  • Male
  • Membranes / drug effects
  • Membranes / metabolism
  • Platelet Activating Factor / antagonists & inhibitors*
  • Platelet Membrane Glycoproteins*
  • Receptors, Cell Surface / metabolism*
  • Receptors, G-Protein-Coupled*
  • Triazines / pharmacology*
  • Triazoles*

Substances

  • Azepines
  • Platelet Activating Factor
  • Platelet Membrane Glycoproteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Triazines
  • Triazoles
  • platelet activating factor receptor
  • WEB 2086