Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats

Dai-Hung Ngo; Kyong-Hwa Kang; BoMi Ryu; Thanh-Sang Vo; Won-Kyo Jung; Hee-Guk Byun; Se-Kwon Kim

doi:10.1016/j.foodchem.2014.11.013

Angiotensin-I converting enzyme inhibitory peptides from antihypertensive skate (Okamejei kenojei) skin gelatin hydrolysate in spontaneously hypertensive rats

Food Chem. 2015 May 1:174:37-43. doi: 10.1016/j.foodchem.2014.11.013. Epub 2014 Nov 8.

Authors

Dai-Hung Ngo¹, Kyong-Hwa Kang¹, BoMi Ryu², Thanh-Sang Vo¹, Won-Kyo Jung³, Hee-Guk Byun⁴, Se-Kwon Kim⁵

Affiliations

¹ Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
² School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
³ Department of Biomedical Engineering, and Center for Marine-integrated Biomedical Technology (BK 21 Plus), Pukyong National University, Busan 608-737, Republic of Korea.
⁴ Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University, Gangneung 210-720, Republic of Korea.
⁵ Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea; Department of Marine-Bio Convergence Science, Specialized Graduate School Science and Technology Convergence, Pukyong National University, Busan 608-739, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.

PMID: 25529649
DOI: 10.1016/j.foodchem.2014.11.013

Abstract

The aim of this study was to investigate antihypertensive effect of bioactive peptides from skate (Okamejei kenojei) skin gelatin. The Alcalase/protease gelatin hydrolysate below 1 kDa (SAP) exhibited the highest angiotensin-I converting enzyme (ACE) inhibition compared to other hydrolysates. SAP can decrease systolic blood pressure significantly in spontaneously hypertensive rats. SAP inhibited vasoconstriction via PPAR-γ expression, activation and phosphorylation of eNOS in lungs. Moreover, the expression levels of endothelin-1, RhoA, α-smooth muscle actin, cleaved caspase 3 and MAPK were decreased by SAP in lungs. Vascularity, muscularization and cellular proliferation in lungs were detected by immunohistochemical staining. Finally, two purified peptides (LGPLGHQ, 720Da and MVGSAPGVL, 829Da) showed potent ACE inhibition with IC50 values of 4.22 and 3.09 μM, respectively. These results indicate that bioactive peptides isolated from skate skin gelatin may serve as candidates against hypertension and could be used as functional food ingredients.

Keywords: Angiotensin converting enzyme inhibition; Antihypertension; PPAR-γ-eNOS; Skate peptides; Spontaneously hypertensive rats.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Angiotensin-Converting Enzyme Inhibitors / administration & dosage*
Angiotensin-Converting Enzyme Inhibitors / chemistry
Animals
Antihypertensive Agents / administration & dosage*
Antihypertensive Agents / chemistry
Blood Pressure / drug effects
Female
Fish Proteins / chemistry*
Gelatin / chemistry*
Humans
Hydrolysis
Hypertension / drug therapy*
Hypertension / enzymology
Male
Peptides / administration & dosage*
Peptides / chemistry
Peptidyl-Dipeptidase A / metabolism
Rats
Rats, Inbred SHR
Skates, Fish*
Skin / chemistry*
Subtilisins / chemistry
Vasoconstriction / drug effects

Substances

Angiotensin-Converting Enzyme Inhibitors
Antihypertensive Agents
Fish Proteins
Peptides
Gelatin
Peptidyl-Dipeptidase A
Subtilisins