Anti-cancer, pharmacokinetics and tumor localization studies of pH-, RF- and thermo-responsive nanoparticles

Int J Biol Macromol. 2015 Mar:74:249-62. doi: 10.1016/j.ijbiomac.2014.11.044. Epub 2014 Dec 17.

Abstract

The curcumin-encapsulated chitosan-graft-poly(N-vinyl caprolactam) nanoparticles containing gold nanoparticles (Au-CRC-TRC-NPs) were developed by ionic cross-linking method. After "optimum RF exposure" at 40 W for 5 min, Au-CRC-TRC-NPs dissipated heat energy in the range of ∼42°C, the lower critical solution temperature (LCST) of chitosan-graft-poly(N-vinyl caprolactam), causing controlled curcumin release and apoptosis to cancer cells. Further, in vivo PK/PD studies on swiss albino mice revealed that Au-CRC-TRC-NPs could be sustained in circulation for a week with no harm to internal organs. The colon tumor localization studies revealed that Au-CRC-TRC-NPs were retained in tumor for a week. These results throw light on their feasibility as multi-responsive nanomedicine for RF-assisted cancer treatment modalities.

Keywords: Chitosan-graft-PNVCL; Curcumin; Gold; RF therapy; Tumor localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / administration & dosage*
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects
  • Caprolactam / chemistry*
  • Cell Line, Tumor
  • Chitosan / chemistry*
  • Curcumin / administration & dosage*
  • Curcumin / pharmacology*
  • Disease Models, Animal
  • Drug Delivery Systems
  • Gold / chemistry
  • Humans
  • Hydrogen-Ion Concentration
  • Mice
  • Nanoparticles / chemistry*
  • Neoplasms / pathology
  • Neoplasms / therapy
  • Particle Size
  • Pulsed Radiofrequency Treatment
  • Thermodynamics
  • Tissue Distribution
  • Tumor Burden / drug effects
  • Tumor Burden / radiation effects

Substances

  • Antineoplastic Agents, Phytogenic
  • Caprolactam
  • Gold
  • Chitosan
  • Curcumin