The mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) and related phylogenetic analyses

Zhengqing Zhang; Xiaoji Wang; Rongzhou Li; Ruijian Guo; Wei Zhang; Wang Song; Chunfeng Hao; Huapeng Wang; Menglou Li

doi:10.1016/j.gene.2014.12.026

The mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) and related phylogenetic analyses

Gene. 2015 Apr 10;560(1):15-24. doi: 10.1016/j.gene.2014.12.026. Epub 2014 Dec 15.

Authors

Zhengqing Zhang¹, Xiaoji Wang², Rongzhou Li², Ruijian Guo¹, Wei Zhang¹, Wang Song¹, Chunfeng Hao¹, Huapeng Wang¹, Menglou Li³

Affiliations

¹ Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
² Forestry Technology Promotion Center of Xian, Xian, Shaanxi 710061, China.
³ Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China. Electronic address: limenglou@hotmail.com.

PMID: 25523091
DOI: 10.1016/j.gene.2014.12.026

Abstract

The complete mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) which consists of 13 PCGs, 22 tRNA genes, two rRNA genes and a non-coding region (D-loop), is sequenced for its nucleotide sequence of 15,878 bp (GenBank: KF811054.1). The genome has a typical gene order which is identical to other Coleoptera species. Except for COI gene generally starts with non-canonical initial codon, all protein-coding genes start with ATN codon and terminate with the stop codon TA(A) or TAG. The secondary structure of rrnL and rrnS consists of 48 helices (contains four newly proposed helices) and 35 helices (contains two newly proposed helices) respectively. All 22 tRNAs in D. helophoroides are predicted to fold into typical cloverleaf secondary structure, except trnS1 (AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. Thirteen protein-coding genes (nucleotide dataset and nucleic acid dataset) of the available species (29 taxa) have been used to infer the phylogenetic relationships among these orders. Tenebrionoidea and Cucujoidea form a sister group, and D. helophoroides is classified into Cucujoidea (Bothrideridae). The study first research on the phylogenetic analyses involving to the D. helophoroides mitogenome, and the results strongly bolster the current morphology-based hypothesis.

Keywords: Dastarcus helophoroides; Mitochondrial genome; Molecular phylogeny; Sequence analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Coleoptera / genetics*
Genome, Mitochondrial*
Molecular Sequence Data
Nucleic Acid Conformation
Phylogeny*
RNA, Ribosomal / genetics
RNA, Transfer / chemistry
RNA, Transfer / genetics
RNA, Untranslated / genetics
Sequence Analysis, DNA*

Substances

RNA, Ribosomal
RNA, Untranslated
RNA, Transfer