Diagnosis of human metapneumovirus in patients hospitalized with acute lower respiratory tract infection using a metal-enhanced fluorescence technique

Ying-Feng Chang; Kuo-Chien Tsao; Yi-Chun Liu; Yu-Chi Chen; Pei-Chun Yu; Yhu-Chering Huang; Chien Chou

doi:10.1016/j.jviromet.2014.12.005

Diagnosis of human metapneumovirus in patients hospitalized with acute lower respiratory tract infection using a metal-enhanced fluorescence technique

J Virol Methods. 2015 Mar:213:151-6. doi: 10.1016/j.jviromet.2014.12.005. Epub 2014 Dec 15.

Authors

Ying-Feng Chang¹, Kuo-Chien Tsao², Yi-Chun Liu³, Yu-Chi Chen⁴, Pei-Chun Yu⁴, Yhu-Chering Huang⁵, Chien Chou⁶

Affiliations

¹ Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan; National Taiwan University, Department of Biochemical Science and Technology, Taipei 10617, Taiwan.
² Linkou Chang Gung Memorial Hospital, Department of Laboratory Medicine, Taoyuan 33305, Taiwan; Chang Gung University, College of Medicine, Department of Medical Biotechnology and Laboratory Science, Taoyuan 33371, Taiwan.
³ Linkou Chang Gung Memorial Hospital, Department of Laboratory Medicine, Taoyuan 33305, Taiwan.
⁴ Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan.
⁵ Linkou Chang Gung Memorial Hospital, Division of Pediatric Infectious Diseases, Taoyuan 33305, Taiwan; Chang Gung University, College of Medicine, Department of Medicine, Taoyuan 33371, Taiwan.
⁶ Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan; Chang Gung University, Healthy Aging Research Center, Taoyuan 33302, Taiwan. Electronic address: cchou01@gamil.com.

PMID: 25522922
DOI: 10.1016/j.jviromet.2014.12.005

Abstract

Human metapneumovirus (hMPV) is a common respiratory tract infection in children. However, conventional immunofluorescence assays (IFAs) for detecting hMPV in respiratory samples have limited reliability with a sensitivity and false-negative predictive value of 58.1% and approximately 17.8%, respectively. In this study, hMPV was measured in 91 clinical respiratory samples (55 sputum and 36 nasopharyngeal aspirate samples), which were obtained from children under three years of age, utilizing our previously developed high-throughput metal-enhanced fluorescence (MEF)-based biosensor (HT-MEFB). The sensitivity of HT-MEFB for hMPV detection in the 91 samples was improved by up to 77.4% compared with that obtained with IFAs, and the specificity of HT-MEFB for hMPV detection was 91.7%. In addition, the specificity and accuracy obtained after the selection of 55 sputum samples as the analyzed specimen reached 92.3% and 90.9%, respectively. Thus, in terms of accuracy, high throughput, and sensitivity, HT-MEFB exhibits considerable potential for hMPV detection in clinical settings.

Keywords: Gold nanoparticles; High-throughput biosensor; Localized surface plasmon resonance; Metal-enhanced fluorescence; Virus detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Child, Preschool
Clinical Laboratory Techniques / methods*
Diagnostic Tests, Routine / methods*
Female
Fluorescence
Hospitalization
Humans
Infant
Infant, Newborn
Male
Metapneumovirus / isolation & purification*
Nasopharynx / virology
Paramyxoviridae Infections / diagnosis*
Paramyxoviridae Infections / virology
Respiratory Tract Infections / diagnosis*
Respiratory Tract Infections / virology
Sensitivity and Specificity
Sputum / virology