Translocation and biotransformation of CuO nanoparticles in rice (Oryza sativa L.) plants

Cheng Peng; Dechao Duan; Chen Xu; Yongsheng Chen; Lijuan Sun; Hai Zhang; Xiaofeng Yuan; Lirong Zheng; Yuanqiang Yang; Jianjun Yang; Xiangjun Zhen; Yingxu Chen; Jiyan Shi

doi:10.1016/j.envpol.2014.12.008

Translocation and biotransformation of CuO nanoparticles in rice (Oryza sativa L.) plants

Environ Pollut. 2015 Feb:197:99-107. doi: 10.1016/j.envpol.2014.12.008. Epub 2014 Dec 15.

Authors

Cheng Peng¹, Dechao Duan², Chen Xu³, Yongsheng Chen⁴, Lijuan Sun⁵, Hai Zhang⁶, Xiaofeng Yuan⁷, Lirong Zheng⁸, Yuanqiang Yang⁹, Jianjun Yang¹⁰, Xiangjun Zhen¹¹, Yingxu Chen¹², Jiyan Shi¹³

Affiliations

¹ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: zjupcsnow@gmail.com.
² Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: duandechao111@163.com.
³ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: smilechenbanban@126.com.
⁴ School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States. Electronic address: yongsheng.chen@ce.gatech.edu.
⁵ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: sunliuliu2012@126.com.
⁶ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: zhangh@zhdec.com.
⁷ College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address: sjyxf.ok@163.com.
⁸ Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zhenglr@ihep.ac.cn.
⁹ Department of Technology, Beijing Construction Engineering Environmental Remediation Co., Ltd., Beijing 100015, China. Electronic address: yangyuanqiang@bceer.com.
¹⁰ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: youngjianjun.cn@gmail.com.
¹¹ Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China. Electronic address: zhenxiangjun@sinap.ac.cn.
¹² Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: yingxuc@gmail.com.
¹³ Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: shijiyan@zju.edu.cn.

PMID: 25521412
DOI: 10.1016/j.envpol.2014.12.008

Abstract

Metal-based nanoparticles (MNPs) may be translocated and biochemically modified in vivo, which may influence the fate of MNPs in the environment. Here, synchrotron-based techniques were used to investigate the behavior of CuO NPs in rice plants exposed to 100 mg/L CuO NPs for 14 days. Micro X-ray fluorescence (μ-XRF) and micro X-ray absorption near edge structure (μ-XANES) analysis revealed that CuO NPs moved into the root epidermis, exodermis, and cortex, and they ultimately reached the endodermis but could not easily pass the Casparian strip; however, the formation of lateral roots provided a potential pathway for MNPs to enter the stele. Moreover, bulk-XANES data showed that CuO NPs were transported from the roots to the leaves, and that Cu (II) combined with cysteine, citrate, and phosphate ligands and was even reduced to Cu (I). CuO NPs and Cu-citrate were observed in the root cells using soft X-ray scanning transmission microscopy (STXM).

Keywords: CuO NPs; Rice; STXM; XAFS; XRF.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Biotransformation
Copper / metabolism*
Metal Nanoparticles / chemistry
Nanoparticles / chemistry
Nanoparticles / metabolism*
Oryza / metabolism*
Plant Leaves
Plant Roots / metabolism
Plants
Synchrotrons

Substances

Copper
cupric oxide