Mesoderm Differentiation from hiPS Cells

Methods Mol Biol. 2016:1357:403-13. doi: 10.1007/7651_2014_162.

Abstract

Human induced pluripotent stem (hiPS) cells are very attractive tools for modeling diseases and regenerative medicine. However, to achieve them, the efficient differentiation methods of hiPS cells into aimed cell type in vitro are necessary. Because mesoderm cells are useful in particular, we have developed the differentiation of mouse embryonic stem (mES) cells into mesoderm cells previously. In this time, these methods were improved for hiPS cells and now human mesoderm cells are able to be obtained efficiently. It is certain that the new methods are applicable to various studies and therapies.

Keywords: Bone; Cartilage; Human induced pluripotent stem (hiPS) cells; Mesoderm; Platelet-derived growth factor receptor α (PDGFRα); Vascular endothelial growth factor receptor 2 (VEGFR2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Differentiation / analysis
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Lineage
  • Cellular Reprogramming Techniques / methods*
  • Cellular Reprogramming*
  • Chondrogenesis
  • Colony-Forming Units Assay
  • Culture Media / pharmacology
  • Fibroblasts / cytology
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Mesoderm / chemistry
  • Mesoderm / cytology*
  • Mice
  • Osteogenesis
  • Receptor, Platelet-Derived Growth Factor alpha / analysis
  • Vascular Endothelial Growth Factor Receptor-2 / analysis

Substances

  • Antigens, Differentiation
  • Culture Media
  • KDR protein, human
  • Receptor, Platelet-Derived Growth Factor alpha
  • Vascular Endothelial Growth Factor Receptor-2