Indirect spectrophotometric determination of BIDA, DISIDA, DTPA and MDP in labelled compounds

T N van der Walt; P P Coetzee; P J Fourie

doi:10.1016/0883-2889(89)90139-1

Indirect spectrophotometric determination of BIDA, DISIDA, DTPA and MDP in labelled compounds

Int J Rad Appl Instrum A. 1989;40(6):525-9. doi: 10.1016/0883-2889(89)90139-1.

Authors

T N van der Walt¹, P P Coetzee, P J Fourie

Affiliation

¹ Department of Chemistry, Rand Afrikaans University, Johannesburg, Republic of South Africa.

PMID: 2551849
DOI: 10.1016/0883-2889(89)90139-1

Abstract

N-(4-(n-butyl)-acetanilide)iminodiacetic acid (BIDA), N-(2,6-diisopropylacetanilide)iminodiacetic acid (DISIDA), diethylenetriaminepentaacetic acid (DTPA) and methylene diphosphonic acid (MDP) are used in labelling kits. The contents of BIDA, DISIDA or MDP of the 99mTc-labelled compounds can be determined (indirectly) spectrophotometrically with copper, eriochrome cyaanine R (ECC) and dodecylethyldimethylammonium bromide (DEDA) in a sodium barbital buffered system at pH 8.5. The calibration curves obey Beer's Law from 0 to 40 micrograms/25 mL for BIDA and DISIDA, 0 to 60 micrograms/25 mL for DTPA and 0 to 100 micrograms/10 mL for MDP.

MeSH terms

Calibration
Imino Acids / analysis*
Organotechnetium Compounds
Pentetic Acid / analysis*
Reagent Kits, Diagnostic
Spectrophotometry / methods
Technetium / analysis*
Technetium Tc 99m Disofenin
Technetium Tc 99m Medronate / analysis*
Technetium Tc 99m Pentetate

Substances

Imino Acids
Organotechnetium Compounds
Reagent Kits, Diagnostic
technetium Tc 99m (para-butyl)iminodiacetic acid
Technetium
Pentetic Acid
Technetium Tc 99m Disofenin
Technetium Tc 99m Pentetate
Technetium Tc 99m Medronate