High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements.
Keywords: bionanotechnology; co-localization; microscopy; nanoparticles.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.