Trajectory-based co-localization measures for nanoparticle-cell interaction studies

Small. 2015 May 6;11(17):2026-31. doi: 10.1002/smll.201401849. Epub 2014 Dec 15.

Abstract

High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements.

Keywords: bionanotechnology; co-localization; microscopy; nanoparticles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Cell Line, Tumor
  • Fluorescent Dyes / chemistry
  • Humans
  • Imaging, Three-Dimensional
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Lysosomes / chemistry
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Nanoparticles / chemistry*
  • Polystyrenes / chemistry
  • Stochastic Processes
  • Time Factors

Substances

  • Fluorescent Dyes
  • Polystyrenes