Chimeric neuraminidase and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus

J Gen Virol. 2015 Apr;96(Pt 4):752-755. doi: 10.1099/jgv.0.000025. Epub 2014 Dec 12.

Abstract

We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here, we showed that the same mutations improved the growth kinetics of a virus containing the A/Vietnam/1203/2004 (H5N1) haemagglutinin and neuraminidase (NA). Total protein yield and NA activity were increased when a chimeric NA was included. These increases indicated that the synergistic effect was due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.

Publication types

  • Editorial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Coculture Techniques
  • Dogs
  • HEK293 Cells
  • Hemagglutinins / genetics
  • Humans
  • Influenza A Virus, H5N1 Subtype / enzymology
  • Influenza A Virus, H5N1 Subtype / genetics*
  • Influenza A Virus, H5N1 Subtype / growth & development*
  • Influenza A Virus, H5N1 Subtype / immunology
  • Influenza Vaccines / genetics*
  • Influenza Vaccines / immunology
  • Madin Darby Canine Kidney Cells
  • Mutation*
  • Neuraminidase / genetics*
  • Viral Proteins / genetics*

Substances

  • Hemagglutinins
  • Influenza Vaccines
  • Viral Proteins
  • influenza virus polymerase basic protein 1
  • Neuraminidase