Acanthamoeba encephalitis: isolation of genotype T1 in mycobacterial liquid culture medium

Rula Azzam; Paul R Badenoch; Michelle J Francis; Charles Fernandez; Penelope J Adamson; Claire Dendle; Ian Woolley; Jenny Robson; Tony M Korman; Maryza Graham

doi:10.1128/JCM.02887-14

Acanthamoeba encephalitis: isolation of genotype T1 in mycobacterial liquid culture medium

J Clin Microbiol. 2015 Feb;53(2):735-9. doi: 10.1128/JCM.02887-14. Epub 2014 Dec 10.

Authors

Affiliations

¹ Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia.
² Department of Ophthalmology, Flinders Medical Centre and Flinders University, Bedford Park, South Australia, Australia.
³ Department of Microbiology, Monash Health, Clayton, Victoria, Australia.
⁴ Department of Microbiology and Infectious Diseases, SA Pathology at Flinders Medical Centre and Flinders University, Bedford Park, South Australia.
⁵ Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Medicine, Monash University, Clayton, Victoria, Australia.
⁶ Sullivan Nicolaides Pathology, Brisbane, Queensland, Australia.
⁷ Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Microbiology, Monash Health, Clayton, Victoria, Australia Department of Medicine, Monash University, Clayton, Victoria, Australia.
⁸ Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Microbiology, Monash Health, Clayton, Victoria, Australia Maryza.Graham@monashhealth.org.

Abstract

We report a case of Acanthamoeba encephalitis diagnosed from an antemortem brain biopsy specimen, where the organism was first isolated in mycobacterial liquid medium and first identified by using a sequence generated by a commercial panfungal sequencing assay. We correlate susceptibility results with clinical outcome.

Publication types

Case Reports

MeSH terms

Acanthamoeba / classification*
Acanthamoeba / genetics
Acanthamoeba / isolation & purification*
Aged
Biopsy
Brain / diagnostic imaging
Brain / parasitology*
Brain / pathology
Central Nervous System Protozoal Infections / diagnosis*
Central Nervous System Protozoal Infections / parasitology
DNA, Protozoan / chemistry
DNA, Protozoan / genetics
Genotype*
Histocytochemistry
Humans
Magnetic Resonance Imaging
Male
Microbiological Techniques
Microscopy
Molecular Sequence Data
Radiography
Sequence Analysis, DNA

Substances

DNA, Protozoan

Associated data

GENBANK/KM015457