The present findings were aimed to develop an effective form of collagen-chitosan film to investigate the wound healing property in Wistar rats. Primarily, the acid-soluble collagen (ASC) isolated, structurally and physicochemically characterized. FT-IR and 1H NMR spectra were confirmed the collagen peptide crosslinks. The molecular weight of ASC subunits was about 86 kDa and 79 kDa, the proline and hydroxyproline content was 13.15% and 7.55%, respectively. In addition, collagen-chitosan films were successfully prepared in a controlled environment and characterized by FT-IR and DSC. In wound healing, the experimental animal model showed the increased wound size reduction in the CChF treated rats (96.25%) when compared to control rats (33.75%) followed by CF treated rats (65%) and ChF (55%). The histological results well supported these findings. It can be concluded that a better and faster epithelization was observed in the CChF group compared to the other groups.
Keywords: Cephalopods; Chitosan; Collagen; Cuttlebone; Wound healing.
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