Abstract
Two new triterpenoid saponins were isolated from the 70% ethanol extract of the rhizome of Anemone amurensis, they are oleanolic acid 28-O-β-d-glucopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl ester (1) and 23,27-dihydroxy oleanolic acid 3-O-α-l-arabinopyranoside (2). The structures of 1 and 2 were elucidated on the basis of chemical and spectral analysis, including 1D and 2D NMR data and HR-ESI-MS. Compounds 1 and 2 were tested for cytotoxicities against three human cancer cell lines (A549, Hep-G2, and MCF-7). Compound 1 showed potent cytotoxicity with IC50 values of 34.76, 41.17, and 28.92 μM, respectively, while compound 2 with IC50>100 μM.
Keywords:
Anemone amurensis; NMR; cytotoxic activity; triterpenoid saponins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anemone / chemistry*
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification*
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Antineoplastic Agents, Phytogenic / pharmacology
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Drug Screening Assays, Antitumor
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology
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Hep G2 Cells
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Humans
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MCF-7 Cells
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Oleanolic Acid / analogs & derivatives
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Oleanolic Acid / chemistry
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Oleanolic Acid / isolation & purification*
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Oleanolic Acid / pharmacology
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Rhizome / chemistry*
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Saponins / chemistry
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Saponins / isolation & purification*
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Saponins / pharmacology
Substances
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23,27-dihydroxy oleanolic acid 3-O-alpha-L-arabinopyranoside
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Antineoplastic Agents, Phytogenic
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Drugs, Chinese Herbal
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Saponins
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oleanolic acid 28-O-beta-D-glucopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl ester
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Oleanolic Acid