Abstract
Three saikosaponins were isolated from the MeOH extract of the roots of Bupleurum falcatum L.: saikosaponins B3 (1); B4 (2); and D (3). Of the three, compound 3 inhibited the interaction of selectins (E, L, and P) and THP-1 cells with IC50 values of 1.8, 3.0 and 4.3 µM, respectively. Also, the aglycone structure 4 of compound 3 showed moderate inhibitory activity on L-selectin-mediated cell adhesion. From these results, we suspect that compound 3 isolated from Bupleurum falcatum roots would be a good candidate for therapeutic strategies to treat inflammation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bupleurum / chemistry*
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Cell Adhesion / drug effects*
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Cell Adhesion Molecules / genetics
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Cell Adhesion Molecules / metabolism
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Cell Line
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Cell Survival / drug effects
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Endothelial Cells / drug effects
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Endothelial Cells / metabolism
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Gene Expression
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Humans
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Immunophenotyping
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Molecular Structure
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Monocytes / drug effects
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Monocytes / metabolism
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Oleanolic Acid / analogs & derivatives*
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Oleanolic Acid / chemistry
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Oleanolic Acid / isolation & purification
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Oleanolic Acid / pharmacology
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Roots / chemistry
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Saponins / chemistry
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Saponins / isolation & purification
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Saponins / pharmacology*
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Selectins / pharmacology*
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Tumor Necrosis Factor-alpha
Substances
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Cell Adhesion Molecules
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Plant Extracts
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Saponins
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Selectins
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Tumor Necrosis Factor-alpha
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Oleanolic Acid
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saikosaponin D