Abstract
The organic matrix of nacre has been reported for its effect on osteogenesis. It was found that PFMG4 (Pinctada fucata mantle gene 4) with an N-terminal signal peptide could be secreted into nacre of Pinctada fucata (P. fucata). Here, we report that PFMG4 is highly expressed in mantle tissue and has high homology with C1q protein in different species. In MC3T3-E1 osteoblast cells, we found that highly expressed PFMG4 could suppress cell proliferation and type I collagen expression, but it could increase alkaline phosphatase activity and mineralized deposition. These results show that PFMG4 has potential ability in enhancing osteoblast differentiation, suggesting a new idea in developing medicine for the therapy of osteoporosis.
Keywords:
MC3T3-E1; Pinctada fucata; Pinctada fucata mantle gene 4 (PFMG4); biomineralization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Factors / genetics
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Biological Factors / isolation & purification
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Biological Factors / metabolism
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Biological Factors / pharmacology*
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Calcification, Physiologic / drug effects
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Calcification, Physiologic / genetics
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Cell Differentiation / drug effects*
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Cell Line
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Cell Proliferation
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Collagen Type I / genetics
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Collagen Type I / metabolism
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Complement C1q / genetics
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Complement C1q / metabolism
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Gene Expression Regulation
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Mice
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Molecular Sequence Data
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Osteoblasts / cytology
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Osteoblasts / drug effects*
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Osteoblasts / metabolism
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Osteogenesis / drug effects
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Osteogenesis / genetics
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Pinctada / chemistry*
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Pinctada / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Signal Transduction
Substances
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Biological Factors
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Collagen Type I
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Complement C1q
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Alkaline Phosphatase