In this study, we determined the complete mitochondrial DNA sequence of the hybrid of Megalobrama amblycephala (♀) × Megalobrama terminalis (♂) for the first time. The complete mitochondrial genome of the hybrid bream was sequenced to be 16,623 bp in size in accordance with the female parent, M. amblycephala. The genome contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). Sequence alignment between the mitochondrial genomes of the hybrid and its female parent showed that a total of 23 mutation sites were found in 10 genes or regions, in particular, six sense mutations in four protein-coding genes (including COX1, ATP6, ND6 and Cytb). The genome information presented here may play an important role in further study on the genetic mechanisms of mitochondrial DNA in hybrids.
Keywords: Hybrid of Megalobrama amblycephala (♀) × Megalobrama terminalis (♂); maternal inheritance; mitochondrial genome; sense mutation.