The ever expanding Central and Eastern European Proteomic Conference (CEEPC) hosted its 8th annual meeting in Vienna, Austria, in July 2014 with resounding success, highlights of which are shared in this report. Tremendous progress in proteomics over the past decade in Central and Eastern Europe continues to rapidly accelerate due to networking across borders as well as access to sophisticated technologies. As the popularity of targeted proteomics in pathogenesis grows to unravel the complexities, so does the use of advanced analytical instrumentation. In addition, development of more sensitive research methodologies and a massive integration of technologies now give optimism to gain better understanding of the structure, functions and isoforms of various proteins as well as their complex interactions in biological systems. This, together with the confidence to qualitatively and/or quantitatively interrogate proteins of interest has led to promising developments for the identification of potential new drug targets for the treatment of various diseases.