A lectin with antifungal activity from the mussel Crenomytilus grayanus

Irina V Chikalovets; Oleg V Chernikov; Mikhail V Pivkin; Valentina I Molchanova; Alina P Litovchenko; Wei Li; Pavel A Lukyanov

doi:10.1016/j.fsi.2014.11.036

A lectin with antifungal activity from the mussel Crenomytilus grayanus

Fish Shellfish Immunol. 2015 Feb;42(2):503-7. doi: 10.1016/j.fsi.2014.11.036. Epub 2014 Dec 5.

Authors

Irina V Chikalovets¹, Oleg V Chernikov², Mikhail V Pivkin³, Valentina I Molchanova³, Alina P Litovchenko⁴, Wei Li⁵, Pavel A Lukyanov³

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia; Far Eastern Federal University, 8, Sukhanova str., Vladivostok 690950, Russia.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia. Electronic address: chernikovoleg@gmail.com.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
⁴ Far Eastern Federal University, 8, Sukhanova str., Vladivostok 690950, Russia.
⁵ College of Food Science and Engineering, Dalian Ocean University, 52, Heishijiaojie, Shahekou District, Dalian, Liaoning, China.

PMID: 25482060
DOI: 10.1016/j.fsi.2014.11.036

Abstract

Lectins (carbohydrate-binding proteins) are well known to actively participate in the defense functions of vertebrates and invertebrates where they play an important role in the recognition of foreign particles. In this study, we investigated of in vitro antifungal activity of lectin from the mussel Crenomytilus grayanus (CGL). Enzyme-linked immunosorbent assay indicated that CGL was predominantly detectable in tissues of mantle and to a lesser degree in the tissues of muscle, hepatopancreas, gill and hemocytes. After challenged by Pichia pastoris the level of CGL was upregulated and reached the maximum level at 12 h post challenge and recovered to the original level at 24 h. The lectin was capable of inhibiting the germination of spores and hyphal growth in the fungi. All these results indicated that CGL is involved in the innate immune response in mollusc animals.

Keywords: Antifungal activity; Crenomytilus grayanus; GalNAc/Gal-specific lectin; Innate immunity; Lectin expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antifungal Agents / metabolism
Galanin / genetics*
Galanin / metabolism
Lectins / genetics*
Lectins / metabolism
Mytilidae / genetics*
Mytilidae / immunology*
Mytilidae / metabolism
Organ Specificity
Pichia / physiology*

Substances

Antifungal Agents
Lectins
Galanin