Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08

Int J Biol Macromol. 2015 Feb:73:236-44. doi: 10.1016/j.ijbiomac.2014.11.028. Epub 2014 Dec 4.

Abstract

The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid. IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained. The structural features and antioxidant activities in vitro of the isolated fractions were evaluated. On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity. The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO). These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.

Keywords: Enzymolysis and acidolysis; Flammulina velutipes SF-08; Intracellular polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology
  • Catalase / metabolism
  • Catalysis
  • Dose-Response Relationship, Drug
  • Flammulina / chemistry*
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / isolation & purification
  • Fungal Polysaccharides / pharmacology
  • Glutathione Peroxidase / metabolism
  • Hydrolysis
  • Lipid Peroxidation
  • Male
  • Mice
  • Oxidation-Reduction
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Fungal Polysaccharides
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase