Arabidopsis cold shock domain protein 2 influences ABA accumulation in seed and negatively regulates germination

Kentaro Sasaki; Myung-Hee Kim; Yuri Kanno; Mitsunori Seo; Yuji Kamiya; Ryozo Imai

doi:10.1016/j.bbrc.2014.11.092

Arabidopsis cold shock domain protein 2 influences ABA accumulation in seed and negatively regulates germination

Biochem Biophys Res Commun. 2015 Jan 2;456(1):380-4. doi: 10.1016/j.bbrc.2014.11.092. Epub 2014 Dec 2.

Authors

Kentaro Sasaki¹, Myung-Hee Kim², Yuri Kanno³, Mitsunori Seo³, Yuji Kamiya³, Ryozo Imai⁴

Affiliations

¹ Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
² Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Center for Plant Aging Research, Institute for Basic Science (IBS), Daegu 711-873, Republic of Korea.
³ RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
⁴ Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan. Electronic address: rzi@affrc.go.jp.

PMID: 25475723
DOI: 10.1016/j.bbrc.2014.11.092

Abstract

The cold shock domain (CSD) is the most conserved nucleic acid binding domain and is distributed from bacteria to animals and plants. CSD proteins are RNA chaperones that destabilize RNA secondary structures to regulate stress tolerance and development. AtCSP2 is one of the four CSD proteins in Arabidopsis and is up-regulated in response to cold. Since AtCSP2 negatively regulates freezing tolerance, it was proposed to be a modulator of freezing tolerance during cold acclimation. Here, we examined the function of AtCSP2 in seed germination. We found that AtCSP2-overexpressing lines demonstrated retarded germination as compared with the wild type, with or without stress treatments. The ABA levels in AtCSP2-overexpressing seeds were higher than those in the wild type. In addition, overexpression of AtCSP2 reduced the expression of an ABA catabolic gene (CYP707A2) and gibberellin biosynthesis genes (GA20ox and GA3ox). These results suggest that AtCSP2 negatively regulates seed germination by controlling ABA and GA levels.

Keywords: ABA; Arabidopsis; Cold shock domain protein; Gibberellin; Seed germination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / chemistry*
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
Cold Shock Proteins and Peptides / metabolism
Cytochrome P-450 Enzyme System / metabolism
Gene Expression Regulation, Plant
Germination*
Gibberellins / chemistry
Mixed Function Oxygenases / metabolism
Plant Proteins
Plants, Genetically Modified
Protein Structure, Tertiary
RNA, Plant / metabolism
RNA-Binding Proteins / metabolism*
Seeds / metabolism*

Substances

Arabidopsis Proteins
Cold Shock Proteins and Peptides
GRP2 protein, Arabidopsis
Gibberellins
Plant Proteins
RNA, Plant
RNA-Binding Proteins
Abscisic Acid
Cytochrome P-450 Enzyme System
Mixed Function Oxygenases
abscisic acid 8'-hydroxylase