Analysis of human T-cell leukemia virus type 1 particles by using cryo-electron tomography

Sheng Cao; José O Maldonado; Iwen F Grigsby; Louis M Mansky; Wei Zhang

doi:10.1128/JVI.02358-14

Analysis of human T-cell leukemia virus type 1 particles by using cryo-electron tomography

J Virol. 2015 Feb;89(4):2430-5. doi: 10.1128/JVI.02358-14. Epub 2014 Dec 3.

Authors

Sheng Cao¹, José O Maldonado¹, Iwen F Grigsby¹, Louis M Mansky², Wei Zhang³

Affiliations

¹ Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
² Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA mansky@umn.edu zhangwei@umn.edu.
³ Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA Characterization Facility, College of Science and Engineering, University of Minnesota, Minneapolis, Minnesota, USA mansky@umn.edu zhangwei@umn.edu.

Abstract

The particle structure of human T-cell leukemia virus type 1 (HTLV-1) is poorly characterized. Here, we have used cryo-electron tomography to analyze HTLV-1 particle morphology. Particles produced from MT-2 cells were polymorphic, roughly spherical, and varied in size. Capsid cores, when present, were typically poorly defined polyhedral structures with at least one curved region contacting the inner face of the viral membrane. Most of the particles observed lacked a defined capsid core, which likely impacts HTLV-1 particle infectivity.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Cryoelectron Microscopy
Electron Microscope Tomography
Human T-lymphotropic virus 1 / ultrastructure*
Humans
Virion / ultrastructure*

Abstract

Publication types

MeSH terms

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