Phytochemical studies of Epilobium angustifolii herba aimed the determination of the active ingredients; antimicrobial activity of the aqueous and ethanolic extracts against human pathogenic bacteria and fungi was also examined. Six flavonoid and 4 phytosterol fractions were identified by thin layer chromatography, while tannins were present in low concentration. It has been shown that not only the ethanolic but also the aqueous extracts had inhibitory effect on certain pathogenic microorganisms, therefore E. angustifolium could be used for the development of external phytotherapic or disinfectant preparations.