Objective: To develop an HPLC method for determination of emodin,chrysophanol and physcion from different medicinal parts of Berchemia lineata.
Methods: Samples were analyzed on Diamonsil ODS C18 (250 mm x 4. 6 mm,5 μm), with the mobile phase consisted of methanol-0. 20% phosphoric acid solution(74: 26). The flow rate was 1.0 mL/min,column temperature was set at 35 °C ,and detection UV wavelength was 254 nm.
Results: The linear range of emodin, chrysophanol and physcion was 0. 00201~ 0. 0804 μg,0. 0066~0. 264 μg and 0. 0124 ~0. 496 μg,with the average recovery was 100. 43% ,101. 29% and 98. 36% ,respectively. The content of total anthraquinones in root was higher than that in taten of Berchemia lineata.
Conclusion: The method is simple,accurate and reliable for quality control of Berchemia lineata.