Visualizing tributyltin (TBT) in bacterial aggregates by specific rhodamine-based fluorescent probes

Xilang Jin; Likai Hao; Mengyao She; Martin Obst; Andreas Kappler; Bing Yin; Ping Liu; Jianli Li; Lanying Wang; Zhen Shi

doi:10.1016/j.aca.2014.10.005

Visualizing tributyltin (TBT) in bacterial aggregates by specific rhodamine-based fluorescent probes

Anal Chim Acta. 2015 Jan 1:853:514-520. doi: 10.1016/j.aca.2014.10.005. Epub 2014 Oct 13.

Authors

Xilang Jin¹, Likai Hao², Mengyao She¹, Martin Obst², Andreas Kappler², Bing Yin³, Ping Liu¹, Jianli Li⁴, Lanying Wang¹, Zhen Shi¹

Affiliations

¹ Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
² Center for Applied Geosciences Eberhard Karls University Tübingen, Hölderlinstr. 12, 72074 Tübingen, Germany.
³ Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China; College of Chemistry, Beijing Normal University, No. 19, Xinjiekouwai Street, Haidian District, Beijing 100875, PR China.
⁴ Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China. Electronic address: lijianli@nwu.edu.cn.

PMID: 25467498
DOI: 10.1016/j.aca.2014.10.005

Abstract

Here we present the first examples of fluorescent and colorimetric probes for microscopic TBT imaging. The fluorescent probes are highly selective and sensitive to TBT and have successfully been applied for imaging of TBT in bacterial Rhodobacter ferrooxidans sp. strain SW2 cell-EPS-mineral aggregates and in cell suspensions of the marine cyanobacterium Synechococcus PCC 7002 by using confocal laser scanning microscopy.

Keywords: Fluorescence imaging; Fluorescent probes; Mapping TBT distribution; Rhodamine; Tributyltin (TBT).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry
Fluorescent Dyes / chemistry*
Microscopy, Confocal*
Models, Molecular
Molecular Conformation
Optical Imaging
Quantum Theory
Rhodamines / chemistry*
Rhodobacter / cytology
Rhodobacter / metabolism*
Schiff Bases / chemistry
Synechococcus / cytology
Synechococcus / metabolism*
Trialkyltin Compounds / chemistry*
Trialkyltin Compounds / metabolism*

Substances

Fluorescent Dyes
Rhodamines
Schiff Bases
Trialkyltin Compounds
tributyltin