Direct transesterification of fresh microalgal cells

Jiao Liu; Yanan Liu; Haitao Wang; Song Xue

doi:10.1016/j.biortech.2014.10.094

Direct transesterification of fresh microalgal cells

Bioresour Technol. 2015 Jan:176:284-7. doi: 10.1016/j.biortech.2014.10.094. Epub 2014 Oct 25.

Authors

Jiao Liu¹, Yanan Liu², Haitao Wang¹, Song Xue³

Affiliations

¹ Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China.
³ Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China. Electronic address: xuesong@dicp.ac.cn.

PMID: 25467001
DOI: 10.1016/j.biortech.2014.10.094

Abstract

Transesterification of lipids is a vital step during the processes of both biodiesel production and fatty acid analysis. By comparing the yields and fatty acid profiles obtained from microalgal oil and dry microalgal cells, the reliability of method for the transesterification of micro-scale samples was tested. The minimum amount of microalgal cells needed for accurate analysis was found to be approximately 300μg dry cells. This direct transesterification method of fresh cells was applied to eight microalgal species, and the results indicate that the efficiency of the developed method is identical to that of conventional method, except for Spirulina whose lipid content is very low, which means the total lipid content should been considered.

Keywords: Direct transesterification; Micro-scale sample; Microalgal cells without dehydration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biofuels*
Biotechnology / methods
Catalysis
Esterification
Lipid Metabolism
Microalgae / metabolism*
Temperature

Substances

Biofuels