Fabrication of PLGA/MWNTs composite electrospun fibrous scaffolds for improved myogenic differentiation of C2C12 cells

Jiazhu Xu; Ya Xie; Hongbo Zhang; Zhaoyang Ye; Wenjun Zhang

doi:10.1016/j.colsurfb.2014.10.041

Fabrication of PLGA/MWNTs composite electrospun fibrous scaffolds for improved myogenic differentiation of C2C12 cells

Colloids Surf B Biointerfaces. 2014 Nov 1:123:907-15. doi: 10.1016/j.colsurfb.2014.10.041. Epub 2014 Oct 30.

Authors

Jiazhu Xu¹, Ya Xie², Hongbo Zhang³, Zhaoyang Ye⁴, Wenjun Zhang⁵

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309 Shanghai 200237, PR China. Electronic address: xujz2009@126.com.
² Complex and Intelligent Research Centre, School of Mechanical and Power Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 401 Shanghai 200237, PR China. Electronic address: hyxieya@163.com.
³ Complex and Intelligent Research Centre, School of Mechanical and Power Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 401 Shanghai 200237, PR China. Electronic address: hbzhang@ecust.edu.cn.
⁴ State Key Laboratory of Bioreactor Engineering, School of Bioengineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 309 Shanghai 200237, PR China. Electronic address: zhaoyangye@ecust.edu.cn.
⁵ Complex and Intelligent Research Centre, School of Mechanical and Power Engineering, East China University of Science and Technology, 130 Mei-Long Road, P. O. Box 401 Shanghai 200237, PR China; Department of Biomedical Engineering, University of Saskatchewan, Canada. Electronic address: zhangwjchris@gmail.com.

PMID: 25466454
DOI: 10.1016/j.colsurfb.2014.10.041

Abstract

Electrically conducting scaffolds have attracted tremendous attention in skeletal muscle tissue engineering. In this paper, poly(lactic-co-glycolic acid) (PLGA)/multi-wall carbon nanotubes (MWNTs) composite fibrous scaffolds were fabricated using the electrospinning technique. The physical properties of the composite fibers were characterized and proliferation and differentiation of C2C12 cells on these scaffolds were examined. It was found that the addition of MWNTs modulated the physical properties of PLGA fibers including morphology, fiber diameter, degradation, tensile strength and electrical conductivity, depending on the amount of MWNTs. These fibrous scaffolds were cytocompatible and supported the proliferation of C2C12 cells. Importantly, C2C12 cells showed more mature myotube formation on PLGA/MWNTs composite fibrous scaffolds compared to PLGA scaffolds. These results indicate that PLGA/MWNTs composite electrospun fibers have great potential in skeletal muscle tissue engineering.

Keywords: C2C12 cells; Carbon nanotubes; Electrical conductivity; Electrospinning; Poly(lactic-co-glycolic acid); Skeletal muscle tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / physiology
Cell Line
Cell Proliferation / drug effects
Lactic Acid / chemistry*
Mice
Muscle, Skeletal / cytology
Nanotubes, Carbon / chemistry*
Polyglycolic Acid / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer
Tissue Engineering / methods
Tissue Scaffolds / chemistry*

Substances

Nanotubes, Carbon
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Lactic Acid