Characterization of trimethylsilyl ethers of iminosugars by gas chromatography-mass spectrometry

S Rodríguez-Sánchez; A I Ruiz-Matute; M L Sanz; A C Soria

doi:10.1016/j.chroma.2014.10.084

Characterization of trimethylsilyl ethers of iminosugars by gas chromatography-mass spectrometry

J Chromatogr A. 2014 Dec 12:1372C:221-227. doi: 10.1016/j.chroma.2014.10.084. Epub 2014 Nov 1.

Authors

S Rodríguez-Sánchez¹, A I Ruiz-Matute¹, M L Sanz¹, A C Soria²

Affiliations

¹ Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain.
² Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: acsoria@iqog.csic.es.

PMID: 25465019
DOI: 10.1016/j.chroma.2014.10.084

Abstract

A combination of gas chromatographic retention data (linear retention indices) on two different stationary phases (100% methyl- and 50% phenylmethylsilicone) and electron impact mass spectrometric relative abundances for characteristic m/z ratios of 12 trimethylsilylated piperidine and pyrrolidine iminosugars are reported. These results have been related to their structural features and applied to the characterization of iminosugar composition of an Aglaonema treubii root extract. Seven iminosugars were detected in this extract, two of them were described for the first time.

Keywords: Aglaonema treubii; Gas chromatography; Iminosugars; Linear retention indices; Mass spectrometry; Trimethylsilylation.