Modeling glands with PTEN deficient cells and microscopic methods for assessing PTEN loss: endometrial cancer as a model

Methods. 2015 May:77-78:31-40. doi: 10.1016/j.ymeth.2014.11.001. Epub 2014 Nov 18.

Abstract

PTEN is an important tumor suppressor gene. Interpreting PTEN deficiency in the appropriate microscopic context of cancer may be important to understand its role in tumor development and progression. This may be particularly relevant in heterogeneous tumors. Here, we discuss the usefulness of 3D cultures in understanding the consequences of PTEN inactivation in tissue architecture. Afterwards, we discuss the role of immunohistochemistry and fluorescent in situ hybridization in assessing PTEN loss in tumors. In this review, endometrial carcinoma is used as a model.

Keywords: Cancer; FISH; Immunohistochemistry; Modeling; PTEN.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Endometrial Neoplasms / genetics
  • Endometrial Neoplasms / metabolism*
  • Endometrial Neoplasms / pathology*
  • Female
  • Humans
  • Mutation / genetics
  • PTEN Phosphohydrolase / deficiency*
  • PTEN Phosphohydrolase / genetics
  • Tissue Culture Techniques / methods*
  • Tumor Suppressor Proteins / deficiency*
  • Tumor Suppressor Proteins / genetics

Substances

  • Tumor Suppressor Proteins
  • PTEN Phosphohydrolase
  • PTEN protein, human