Tetracycline-inducible gene expression system in Leishmania mexicana

Natalya Kraeva; Aygul Ishemgulova; Julius Lukeš; Vyacheslav Yurchenko

doi:10.1016/j.molbiopara.2014.11.002

Tetracycline-inducible gene expression system in Leishmania mexicana

Mol Biochem Parasitol. 2014 Nov;198(1):11-3. doi: 10.1016/j.molbiopara.2014.11.002. Epub 2014 Nov 22.

Authors

Natalya Kraeva¹, Aygul Ishemgulova¹, Julius Lukeš², Vyacheslav Yurchenko³

Affiliations

¹ Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic.
² Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice (Budweis), Czech Republic; Faculty of Sciences, University of South Bohemia, 370 05 České Budějovice (Budweis), Czech Republic; Canadian Institute for Advanced Research, Toronto, ON M5G 1Z8, Canada.
³ Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budějovice (Budweis), Czech Republic. Electronic address: vyacheslav.yurchenko@osu.cz.

PMID: 25461484
DOI: 10.1016/j.molbiopara.2014.11.002

Abstract

Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.

Keywords: Gene expression; Leishmania mexicana; Tet-inducible system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression*
Humans
Leishmania mexicana / drug effects*
Leishmania mexicana / genetics*
Leishmania mexicana / metabolism
Leishmaniasis, Cutaneous / parasitology*
Protozoan Proteins / genetics*
Protozoan Proteins / metabolism
Tetracycline / pharmacology*

Substances

Protozoan Proteins
Tetracycline