Influence of organic loading rate and solid retention time on polyhydroxybutyrate production from hybrid poplar hydrolysates using mixed microbial cultures

Jing Dai; Karol Gliniewicz; Matthew L Settles; Erik R Coats; Armando G McDonald

doi:10.1016/j.biortech.2014.10.049

Influence of organic loading rate and solid retention time on polyhydroxybutyrate production from hybrid poplar hydrolysates using mixed microbial cultures

Bioresour Technol. 2015 Jan:175:23-33. doi: 10.1016/j.biortech.2014.10.049. Epub 2014 Oct 17.

Authors

Jing Dai¹, Karol Gliniewicz², Matthew L Settles², Erik R Coats³, Armando G McDonald⁴

Affiliations

¹ Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, ID 83844-1132, United States.
² Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
³ Department of Civil Engineering, University of Idaho, Moscow, ID 83844, United States.
⁴ Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, ID 83844-1132, United States. Electronic address: armandm@uidaho.edu.

PMID: 25459800
DOI: 10.1016/j.biortech.2014.10.049

Abstract

The aim of this study was to investigate the potential of using wood hydrolysates (enzymatically hydrolyzed from hybrid poplar) as substrate to produce polyhydroxybutyrate (PHB) using mixed microbial cultures. The optimal operational conditions for fed-batch bioreactors were 4d solid retention time with an organic loading rate of 2.5g/Ld. The maximum PHB accumulated was 27% of cell dry weight with a yield of 0.32g/g (g PHB produced per g sugars consumed). Microbial community analysis was done at the genus level by 16S rRNA sequencing on an Illumina system and community evolution was observed among different samples and initial seed. Actinobacteria, Alpha- and Beta-proteobacteria were found to be the dominant groups in all the bioreactors. Several PHB-storing microorganisms were characterized belonging to Alpha- and Beta-proteobacteria.

Keywords: 16S rRNA sequencing; Mixed microbial cultures; Polyhydroxybutyrate; Wood hydrolysates.

MeSH terms

Biomass
Bioreactors / microbiology*
Hydroxybutyrates / chemical synthesis*
Populus / chemistry*
Proteobacteria*
Wood / chemistry

Substances

Hydroxybutyrates