Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria

Jana Klimentová; Jiří Stulík

doi:10.1016/j.micres.2014.09.006

Methods of isolation and purification of outer membrane vesicles from gram-negative bacteria

Microbiol Res. 2015 Jan:170:1-9. doi: 10.1016/j.micres.2014.09.006. Epub 2014 Oct 14.

Authors

Jana Klimentová¹, Jiří Stulík²

Affiliations

¹ Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. Electronic address: jana.klimentova@unob.cz.
² Department of Molecular Pathology and Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic. Electronic address: jiri.stulik@unob.cz.

PMID: 25458555
DOI: 10.1016/j.micres.2014.09.006

Abstract

Outer membrane vesicles secreted by gram-negative bacteria play an important role in bacterial physiology as well as in virulence and host-pathogen interaction. Isolated vesicles of some bacteria have also been studied for their immunomodulatory potential in the vaccine development. However, the production of vesicles in sufficient amount, purity and reproducibility remains a critical challenge for subsequent analyses in most bacteria. In the present review methods of production, isolation, purification and quantification of outer membrane vesicles are summarized and discussed.

Keywords: Bacterial secretion; Gram-negative bacteria; Host–pathogen interaction; Outer membrane vesicles; Vaccine development.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Cell Fractionation / methods*
Cell Wall* / immunology
Cell Wall* / metabolism
Genetic Engineering
Gram-Negative Bacteria* / genetics
Gram-Negative Bacteria* / immunology
Gram-Negative Bacteria* / metabolism
Secretory Vesicles* / immunology
Secretory Vesicles* / metabolism
Stress, Physiological
Subcellular Fractions