Nanocellulose-alginate hydrogel for cell encapsulation

Carbohydr Polym. 2015 Feb 13:116:223-8. doi: 10.1016/j.carbpol.2014.07.059. Epub 2014 Aug 4.

Abstract

TEMPO-oxidized bacterial cellulose (TOBC)-sodium alginate (SA) composites were prepared to improve the properties of hydrogel for cell encapsulation. TOBC fibers were obtained using a TEMPO/NaBr/NaClO system at pH 10 and room temperature. The fibrillated TOBCs mixed with SA were cross-linked in the presence of Ca(2+) solution to form hydrogel composites. The compression strength and chemical stability of the TOBC/SA composites were increased compared with the SA hydrogel, which indicated that TOBC performed an important function in enhancing the structural, mechanical and chemical stability of the composites. Cells were successfully encapsulated in the TOBC/SA composites, and the viability of cells was investigated. TOBC/SA composites can be a potential candidate for cell encapsulation engineering.

Keywords: Alginate; Bacterial cellulose; Cell encapsulation; TEMPO-oxidization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry*
  • Animals
  • Cell Proliferation
  • Cell Survival
  • Cellulose / biosynthesis
  • Cellulose / chemistry*
  • Compressive Strength
  • Cyclic N-Oxides / chemistry
  • Gluconacetobacter xylinus* / metabolism
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Hydrogels / chemistry*
  • Mice
  • Microscopy / methods
  • NIH 3T3 Cells
  • Nanocomposites / chemistry*
  • Nanocomposites / ultrastructure
  • Oxidants / chemistry
  • Oxidation-Reduction
  • Polysaccharides, Bacterial / biosynthesis
  • Polysaccharides, Bacterial / chemistry*
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Alginates
  • Cyclic N-Oxides
  • Hexuronic Acids
  • Hydrogels
  • Oxidants
  • Polysaccharides, Bacterial
  • Glucuronic Acid
  • Cellulose
  • TEMPO