'Click' glycosylation of peptides through cysteine propargylation and CuAAC

Bioorg Med Chem. 2014 Dec 1;22(23):6672-6683. doi: 10.1016/j.bmc.2014.09.056. Epub 2014 Oct 27.

Abstract

'Click' glycosylation of cysteine-containing peptides were carried out in good yield by Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC). For that peptides were functionalized though direct propargylation of the cysteine residue allowing their use in CuAAC with suitable free or protected azido sugars of gluco, manno and galacto configuration. Among these free and protected glycopeptides a series of 'glycoRGD' peptides were obtained and submitted to in vitro platelet aggregation tests, showing that the pseudoglycosylation of the adhesion sequence lowers the IC50 value and thus could improve the in vivo pharmacokinetic properties.

Keywords: Click chemistry; CuAAC; Glycoconjugate; Peptide; RGD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry
  • Azides / chemistry
  • Click Chemistry*
  • Copper / chemistry
  • Cyclization
  • Cysteine / chemistry*
  • Glycosylation
  • Humans
  • Molecular Structure
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Pargyline / analogs & derivatives
  • Pargyline / chemistry*
  • Peptides / chemical synthesis*
  • Peptides / chemistry
  • Peptides / pharmacology
  • Platelet Aggregation / drug effects

Substances

  • Alkynes
  • Azides
  • Organometallic Compounds
  • Peptides
  • Copper
  • Pargyline
  • Cysteine