Revisiting an agar-based plate method: what the static biofilm method can offer for biofilm research

Terhi Oja; Brianna Blomqvist; Kelli Buckingham-Meyer; Darla Goeres; Pia Vuorela; Adyary Fallarero

doi:10.1016/j.mimet.2014.10.008

Revisiting an agar-based plate method: what the static biofilm method can offer for biofilm research

J Microbiol Methods. 2014 Dec:107:157-60. doi: 10.1016/j.mimet.2014.10.008.

Authors

Terhi Oja¹, Brianna Blomqvist, Kelli Buckingham-Meyer, Darla Goeres, Pia Vuorela, Adyary Fallarero

Affiliation

¹ Pharmaceutical Sciences, Department of Biosciences, Abo Akademi University, Turku, Finland.

PMID: 25455020
DOI: 10.1016/j.mimet.2014.10.008

Abstract

The development of biofilms in static plates was monitored. Glass coupons were placed on agar covered with filter paper, which was inoculated with suspended bacteria. The viable cell density, biofilms matrix and biomass were quantified. The method is excellent for adhesion and material studies, due to its simplicity and flexibility.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agar*
Bacteriological Techniques*
Biofilms / growth & development*
Culture Media*
Research

Substances

Culture Media
Agar