Validation of reference genes in the feline endometrium

Reprod Biol. 2014 Dec;14(4):302-6. doi: 10.1016/j.repbio.2014.04.003. Epub 2014 Apr 30.

Abstract

The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM). According to NormFinder and BestKeeper, ACTB (at all examined primer concentrations) followed by RPS7 and CYC were the most stable genes. Based on geNorm results at least two genes from among RPS7, RPL30, ACTB or YWHAZ should be chosen for Real Time-PCR result normalization.

Keywords: Domestic cat; Endometrium; Reference gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Cats / genetics*
  • DNA Primers / genetics
  • Endometrium / metabolism*
  • Female
  • Genes / genetics*
  • Genetic Markers / genetics*
  • Models, Animal*
  • Real-Time Polymerase Chain Reaction / standards
  • Real-Time Polymerase Chain Reaction / veterinary*
  • Software
  • Statistics, Nonparametric

Substances

  • DNA Primers
  • Genetic Markers