Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Monika M Kaczmarek; Kamil Krawczynski; Joanna Najmula; Zaneta P Reliszko; Malgorzata Sikora; Zdzislaw Gajewski

doi:10.1016/j.repbio.2014.06.003

Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Reprod Biol. 2014 Dec;14(4):293-7. doi: 10.1016/j.repbio.2014.06.003. Epub 2014 Jul 9.

Authors

Monika M Kaczmarek¹, Kamil Krawczynski², Joanna Najmula², Zaneta P Reliszko², Malgorzata Sikora², Zdzislaw Gajewski³

Affiliations

¹ Laboratory of Molecular Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland; Department for Large Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Nowoursynowska 100, 02-797 Warsaw, Poland. Electronic address: m.kaczmarek@pan.olsztyn.pl.
² Laboratory of Molecular Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
³ Department for Large Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Nowoursynowska 100, 02-797 Warsaw, Poland.

PMID: 25454496
DOI: 10.1016/j.repbio.2014.06.003

Abstract

The objective of this study was to determine the expression profiles of leukemia inhibitory factor (LIF) and its receptor (LIFR), interleukin 6 receptor (IL6R), tumor protein p53 (TP53) and B-cell CLL/lymphoma 2 (BCL2) in the porcine endometrium on selected days of the estrous cycle and pregnancy. Time- and reproductive status (estrous cycle/pregnancy)-specific patterns of expression were identified for all investigated genes. The most pronounced changes were seen on Days 12 and 14 of pregnancy when maternal recognition of pregnancy and implantation, respectively, occurs in pigs.

Keywords: BCL2; IL6R; LIF; LIFR; Pig; TP53.

Copyright © 2014 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Analysis of Variance
Animals
Endometrium / metabolism
Endometrium / physiology*
Estrous Cycle / metabolism
Estrous Cycle / physiology*
Female
Gene Expression Profiling / veterinary
Gene Expression Regulation, Developmental / physiology*
Leukemia Inhibitory Factor / metabolism*
Pregnancy
Proto-Oncogene Proteins c-bcl-2 / metabolism
Real-Time Polymerase Chain Reaction
Receptors, Interleukin-6 / metabolism
Receptors, OSM-LIF / metabolism
Signal Transduction / physiology*
Swine / metabolism
Swine / physiology*
Tumor Suppressor Protein p53 / metabolism

Substances

Leukemia Inhibitory Factor
Proto-Oncogene Proteins c-bcl-2
Receptors, Interleukin-6
Receptors, OSM-LIF
Tumor Suppressor Protein p53