Targeted analytical toxicology: simultaneous determination of 17α-ethynylestradiol and the estrogen-induced vitellogenin biomarker

Environ Int. 2015 Jan:74:119-24. doi: 10.1016/j.envint.2014.10.015. Epub 2014 Oct 23.

Abstract

In most toxicological studies, chemical concentrations and its biological/molecular response (biomarker) levels are often quantified separately. We report a method, named as Targeted Analytical Toxicology (TAT), for the simultaneous determination of levels of chemicals and protein biomarkers in organisms by UPLC/ESI-MS/MS on the basis of targeted proteomics. The TAT method is successfully applied to detect the concentrations of 17α-ethynylestradiol (EE2) and EE2-induced vitellogenin (a biomarker for estrogens) in male zebrafish exposed to EE2. It is expected for TAT to have broad application in toxicology, especially in environmental toxicology, due to its high sensitivity, accuracy, reproducibility, high throughput, and the savings of time, cost and labor.

Keywords: 17α-Ethynylestradiol; Environmental toxicology; Protein biomarker; Simultaneous determination; Targeted analytical toxicology; Vitellogenin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Biomarkers / metabolism
  • Chromatography, Liquid / methods
  • Ecotoxicology / methods
  • Environmental Pollutants / analysis*
  • Environmental Pollutants / metabolism
  • Estrogens / analysis*
  • Ethinyl Estradiol / analysis*
  • Male
  • Proteomics / methods*
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization / methods
  • Tandem Mass Spectrometry / methods*
  • Vitellogenins / analysis*
  • Vitellogenins / biosynthesis
  • Zebrafish / metabolism

Substances

  • Biomarkers
  • Environmental Pollutants
  • Estrogens
  • Vitellogenins
  • Ethinyl Estradiol