Internal capsule stroke in the common marmoset

S Puentes; T Kaido; T Hanakawa; N Ichinohe; T Otsuki; K Seki

doi:10.1016/j.neuroscience.2014.10.015

Internal capsule stroke in the common marmoset

Neuroscience. 2015 Jan 22:284:400-411. doi: 10.1016/j.neuroscience.2014.10.015. Epub 2014 Oct 20.

Authors

S Puentes¹, T Kaido², T Hanakawa³, N Ichinohe⁴, T Otsuki⁵, K Seki⁶

Affiliations

¹ Department of Neurophysiology, National Institute of Neuroscience, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan. Electronic address: sandra@ncnp.go.jp.
² Department of Neurosurgery, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8551, Japan. Electronic address: kaido@ncnp.go.jp.
³ Department of Advanced Neuroimaging, Integrative Brain Image Center, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8551, Japan. Electronic address: hanakawa@ncnp.go.jp.
⁴ Department of Ultrastructural Research, National Institute of Neuroscience, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan. Electronic address: nichino@ncnp.go.jp.
⁵ Department of Neurosurgery, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8551, Japan. Electronic address: otsukit@ncnp.go.jp.
⁶ Department of Neurophysiology, National Institute of Neuroscience, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan. Electronic address: seki@ncnp.go.jp.

PMID: 25453768
DOI: 10.1016/j.neuroscience.2014.10.015

Abstract

White matter (WM) impairment and motor deficit after stroke are directly related. However, WM injury mechanisms and their relation to motor disturbances are still poorly understood. In humans, the anterior choroidal artery (AChA) irrigates the internal capsule (IC), and stroke to this region can induce isolated motor impairment. The goal of this study was to analyze whether AChA occlusion can injure the IC in the marmoset monkey. The vascular distribution of the marmoset brain was examined by colored latex perfusion and revealed high resemblance to the human brain anatomy. Next, a new approach to electrocoagulate the AChA was developed and chronic experiments showed infarction compromising the IC on magnetic resonance imaging (MRI) scanning (day 4) and histology (day 11). Behavioral analysis was performed using a neurologic score previously developed and our own scoring method. Marmosets showed a decreased score that was still evident at day 10 after AChA electrocoagulation. We developed a new approach able to induce damage to the marmoset IC that may be useful for the detailed study of WM impairment and behavioral changes after stroke in the nonhuman primate.

Keywords: anterior choroidal artery; internal capsule; motor impairment; nonhuman primate; white matter stroke.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Callithrix* / anatomy & histology
Cerebral Arteries / anatomy & histology
Disease Models, Animal*
Humans
Internal Capsule* / blood supply
Internal Capsule* / pathology
Magnetic Resonance Imaging
Movement Disorders / physiopathology
Neurosurgical Procedures
Severity of Illness Index
Stroke* / pathology
Stroke* / physiopathology