Abstract
We were able to partially purify a polar digitalis-like factor from rat and bovine hypothalami based on the capacity to inhibit [3H]ouabain binding to intact human erythrocytes. This factor was characterized in reference to the digitalis-like factor that we have isolated and reported on. Hypothalamic factor shared digitalis-like activities and physicochemical properties with the one derived from human urine and mammalian plasma. These findings strongly suggest that a polar digitalis-like factor identical to the circulatory factor does exist in mammalian hypothalamus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding, Competitive
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Blood Proteins / isolation & purification*
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Blood Proteins / pharmacology
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Cardenolides
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Cattle
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Chromatography, High Pressure Liquid
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Digoxin*
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Dogs
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Erythrocytes / metabolism
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Humans
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Hypothalamus / physiology*
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Kidney / enzymology
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Kinetics
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Ouabain / blood
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Rats
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Rubidium / metabolism
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Saponins*
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Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors*
Substances
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Blood Proteins
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Cardenolides
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Saponins
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digoxin-like factors
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Ouabain
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Digoxin
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Sodium-Potassium-Exchanging ATPase
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Rubidium