Studies on cytotoxic pregnane sapogenins from Cynanchum wilfordii

Fitoterapia. 2015 Mar:101:107-16. doi: 10.1016/j.fitote.2014.10.014. Epub 2014 Nov 13.

Abstract

To investigate their cytotoxicity, seventeen C21-steroidal pregnane sapogenins 1-17 were isolated from the hydrolytic extracts of the roots of Cynanchum wilfordii. Among them, sapogenins 1-7 are new compounds, whose structures were determined by extensive analysis of spectroscopic data and X-ray crystallographic analysis. Especially, sapogenins with salicyl or vanilloyl group, and a new aberrant pregnane skeleton with ether linkage between C-12 and C-20 were found for the first time. Compound 1 revealed significant cytotoxicities on HL-60 (IC50 6.72μM) and MCF-7 (IC50 2.89μM), and compounds 14 and 15 also revealed strong inhibitory activities against K-562 (IC50 6.72μM) or MCF-7 (IC50 2.49μM), respectively.

Keywords: Antitumor; Asclepiadaceae; C(21)-steroidal sapogenins; Cynanchum wilfordii; Cytotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cynanchum / chemistry*
  • HL-60 Cells
  • Humans
  • K562 Cells
  • MCF-7 Cells
  • Molecular Structure
  • Plant Roots / chemistry
  • Pregnanes / isolation & purification
  • Pregnanes / pharmacology*
  • Sapogenins / isolation & purification
  • Sapogenins / pharmacology*

Substances

  • Pregnanes
  • Sapogenins