Structural features and antitumor activity of a purified polysaccharide extracted from Sargassum horneri

Int J Biol Macromol. 2015 Feb:73:124-30. doi: 10.1016/j.ijbiomac.2014.10.056. Epub 2014 Nov 20.

Abstract

A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78×10(5) Da and composed of T-D-Glcp, 1,3-D-Glcp, 1,6-D-Glcp and 1,3,6-D-Glcp in a molar percentage of 1.00:4.17:1.17:0.89, respectively. Based on the results from chemical analysis, NMR, and SHPSA was determined to be a glucan with β-(1→6) side chains linked to a β-(1→3) backbone with relatively few branch points. Moreover, SHPSA could inhibit the growth of human colon cancer DLD cells in a dose-dependent manner by inducing the apoptosis of DLD cells. So, SHPSA was promising for future use as a natural antitumor agent.

Keywords: Anti-tumor activity; Sargassum horneri polysaccharide; Structural characterization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Humans
  • Methylation
  • Molecular Weight
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Extracts / chemistry*
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Sargassum / chemistry*

Substances

  • Antineoplastic Agents
  • Plant Extracts
  • Polysaccharides