Anti-loxoscelic horse serum produced against a recombinant dermonecrotic protein of Brazilian Loxosceles intermedia spider neutralize lethal effects of Loxosceles laeta venom from Peru

Toxicon. 2015 Jan:93:37-40. doi: 10.1016/j.toxicon.2014.10.023. Epub 2014 Oct 30.

Abstract

In this work, an anti-loxoscelic serum was produced by immunizing horses with a recombinant dermonecrotic protein from Loxosceles intermedia (rLiD1). Anti-rLiD1 antibodies were able to recognize different species of Loxosceles venoms by Western Blot and ELISA. The efficacy of anti-rLiD1 serum against the toxic effects of Loxosceles laeta (Peru) venom was tested, showing that anti-rLiD1 serum can neutralize those effects. This study confirms that recombinant proteins can be good candidates to replace crude venoms for antivenom production.

Keywords: Antivenom; Immunization; Loxosceles; Neutralization; Recombinant protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antivenins / immunology*
  • Antivenins / pharmacology
  • Blotting, Western
  • Brazil
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Horses
  • Neutralization Tests
  • Peru
  • Phosphoric Diester Hydrolases / analysis
  • Phosphoric Diester Hydrolases / chemistry*
  • Phosphoric Diester Hydrolases / metabolism*
  • Recombinant Proteins / metabolism*
  • Species Specificity
  • Spider Venoms / chemistry*
  • Spider Venoms / enzymology

Substances

  • Antivenins
  • Recombinant Proteins
  • Spider Venoms
  • loxosceles venom
  • Phosphoric Diester Hydrolases
  • sphingomyelin phosphodiesterase D