A modular zinc finger adaptor accelerates the covalent linkage of proteins at specific locations on DNA nanoscaffolds

Eiji Nakata; Huyen Dinh; Tien Anh Ngo; Masayuki Saimura; Takashi Morii

doi:10.1039/c4cc08167f

A modular zinc finger adaptor accelerates the covalent linkage of proteins at specific locations on DNA nanoscaffolds

Chem Commun (Camb). 2015 Jan 21;51(6):1016-9. doi: 10.1039/c4cc08167f.

Authors

Eiji Nakata¹, Huyen Dinh, Tien Anh Ngo, Masayuki Saimura, Takashi Morii

Affiliation

¹ Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan. t-morii@iae.kyoto-u.ac.jp.

PMID: 25447990
DOI: 10.1039/c4cc08167f

Abstract

A modular adaptor consisting of a sequence-specific DNA binding zinc finger protein and a self-ligating protein-tag was developed to expedite efficient formation of a covalent linkage between an individual protein molecule and the programmed address modified with a tag-substrate on the DNA nanostructure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / metabolism*
DNA / chemistry
DNA / metabolism*
DNA-Binding Proteins / chemistry*
Microscopy, Atomic Force
Nanostructures / chemistry*
Zinc Fingers*

Substances

Adaptor Proteins, Signal Transducing
DNA-Binding Proteins
DNA