Improvement in the DNA sequencing of genomes bearing long repeated elements

Antony T Vincent; Brian Boyle; Nicolas Derome; Steve J Charette

doi:10.1016/j.mimet.2014.10.016

Improvement in the DNA sequencing of genomes bearing long repeated elements

J Microbiol Methods. 2014 Dec:107:186-8. doi: 10.1016/j.mimet.2014.10.016.

Authors

Antony T Vincent¹, Brian Boyle, Nicolas Derome, Steve J Charette

Affiliation

¹ Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, Canada.

PMID: 25447886
DOI: 10.1016/j.mimet.2014.10.016

Abstract

When using next-generation sequencing methods, obtaining a single and complete bacterial chromosomic sequence may be challenging especially for bacteria harboring multiple repeated sequences. Here we present a one-step procedure to optimize the sequencing process in order to obtain a single chromosomic scaffold.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Bacterial
Computational Biology / methods
Genome, Bacterial*
Genomics / methods
High-Throughput Nucleotide Sequencing* / methods
High-Throughput Nucleotide Sequencing* / standards
Repetitive Sequences, Nucleic Acid*