C/EBPα controls mast cell function

Kazumi Kasakura; Kyoko Takahashi; Tomoko Itoh; Akira Hosono; Satoshi Nunomura; Chisei Ra; Yoshika Momose; Kikuji Itoh; Chiharu Nishiyama; Shuichi Kaminogawa

doi:10.1016/j.febslet.2014.10.036

C/EBPα controls mast cell function

FEBS Lett. 2014 Dec 20;588(24):4645-53. doi: 10.1016/j.febslet.2014.10.036. Epub 2014 Nov 11.

Authors

Affiliations

¹ College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan; Atopy (Allergy) Research Center, Juntendo University School of Medicine, Bunkyo, Tokyo, Japan; Department of Biological Science and Technology, Tokyo University of Science, Katsushika, Tokyo, Japan.
² College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. Electronic address: ktaka@brs.nihon-u.ac.jp.
³ College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
⁴ Department of Molecular Cell Immunology and Allergology, Nihon University School of Medicine, Itabashi, Tokyo, Japan.
⁵ Department of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, Japan.
⁶ Atopy (Allergy) Research Center, Juntendo University School of Medicine, Bunkyo, Tokyo, Japan; Department of Biological Science and Technology, Tokyo University of Science, Katsushika, Tokyo, Japan.

PMID: 25447519
DOI: 10.1016/j.febslet.2014.10.036

Abstract

CCAAT/enhancer binding protein alpha (C/EBPα) is a transcription factor that influences immune cell fate and differentiation. However, the effect of C/EBPα on mast cells is not fully understood. In this study, we showed that C/EBPα suppressed granule formation in mast cells and increased macrophage inflammatory protein (MIP)-2 production from mast cells upon bacterial stimulation. These results indicate that C/EBPα regulates the balance between the allergic response and the innate immune response of mast cells. Furthermore, we showed that stimulation of mast cells with the Lactobacillus casei JCM1134(T) strain during late differentiation up-regulated C/EBPα expression in differentiated mast cells. This suggests that intestinal commensal bacteria modulate C/EBPα expression and thereby regulate mast cell function.

Keywords: Allergy; CCAAT/enhancer binding protein alpha; Commensal bacteria; Innate immunity; Mast cell; Terminal differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow Cells / cytology
CCAAT-Enhancer-Binding Protein-alpha / genetics
CCAAT-Enhancer-Binding Protein-alpha / metabolism*
Cell Differentiation
Chemokine CXCL2 / biosynthesis
Female
Gene Expression Regulation
Lacticaseibacillus casei / physiology
Mast Cells / cytology*
Mast Cells / immunology
Mast Cells / metabolism*
Mast Cells / microbiology
Mice

Substances

CCAAT-Enhancer-Binding Protein-alpha
Chemokine CXCL2
Cxcl2 protein, mouse