Apoptotic primary normal human dermal fibroblasts for in vitro models of fibrosis

Elena García-Gareta; Nivedita Ravindran; Julian F Dye

doi:10.1016/j.ab.2014.10.009

Apoptotic primary normal human dermal fibroblasts for in vitro models of fibrosis

Anal Biochem. 2015 Feb 1:470:22-4. doi: 10.1016/j.ab.2014.10.009. Epub 2014 Oct 22.

Authors

Elena García-Gareta¹, Nivedita Ravindran², Julian F Dye²

Affiliations

¹ RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood HA6 2RN, UK. Electronic address: garciae@raft.ac.uk.
² RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood HA6 2RN, UK.

PMID: 25447464
DOI: 10.1016/j.ab.2014.10.009

Abstract

Recent studies show that apoptosis affects surrounding tissue, playing a role in diseases such as fibrosis, a significant global disease burden. Elucidating the mechanisms by which the different apoptotic cells present during fibrotic wound healing affect their environment would enable development of new therapies. We describe here a simple, rapid, and cost-effective method for inducing apoptosis of primary normal human dermal fibroblasts without affecting the overall cell viability of the population. Such population could be used for in vitro models of fibrotic wound healing in co-culture with other cells involved in this process to study events such as apoptosis-induced proliferation.

Keywords: Apoptosis; Cell viability; Ethanol; Fibrosis; Primary normal human dermal fibroblasts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Cell Proliferation
Cost-Benefit Analysis
Cytological Techniques / economics
Cytological Techniques / methods*
Fibroblasts / cytology*
Fibroblasts / pathology
Fibrosis
Humans
Skin / cytology*
Time Factors